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The 2nd reaction cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to produce ferric enzyme, NO, citrulline, and water as products (Fig. 9) - UV/Vis/NIR. As drawn in Fig - https://hubpages.com/@olisclarity1. 9, the reaction might also include electron transfer from H4B to the Fe, IIO2 intermediate
l9 As displayed in Fig. 9, this might be because the H4B radical serve as an electron sink in a later point in the reaction, so as to allow NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.